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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs <t>by</t> <t>HUVECs</t> and <t>BMSCs</t>
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live dead assays bone marrow stromal cells bmscs  (Beijing Solarbio Science)
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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs <t>by</t> <t>HUVECs</t> and <t>BMSCs</t>
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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs <t>by</t> <t>HUVECs</t> and <t>BMSCs</t>
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rat bone marrow stromal cells (bmscs)  (Procell Inc)
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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs <t>by</t> <t>HUVECs</t> and <t>BMSCs</t>
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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs <t>by</t> <t>HUVECs</t> and <t>BMSCs</t>
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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs <t>by</t> <t>HUVECs</t> and <t>BMSCs</t>
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Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs by HUVECs and BMSCs

Journal: Journal of Nanobiotechnology

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair

doi: 10.1186/s12951-025-03596-4

Figure Lengend Snippet: Identification of sEVs of ADSCs cultured by 3D coaxial bioprinting. ( A ) Schematic diagram of cellular microfiber structure fabricated based on coaxial 3D bioprinting. ( B ) The morphology of 2D-sEVs and 3D-sEVs evaluated by TEM. ( C ) NTA analysis showing the size distribution of 2D-sEVs and 3D-sEVs. ( D ) Western blot analysis showing expression levels of sEVs markers CD63, CD81 and TSG101 in 2D-sEVs and 3D-sEVs. ( E ) Laser confocal microscopy images showing the internalization of fluorescently labeled 2D-sEVs and 3D-sEVs by HUVECs and BMSCs

Article Snippet: Bone marrow stromal cells (BMSCs) (Solarbio, China) or human umbilical vein endothelial cells (HUVECs) (Gibco, USA) were seeded at a density of 1 × 104 cells/well in 96-well plates and cultured in the extract liquids of HAMA-CPC, HAMA-CPC@2D-sEVs, or HAMA-CPC@3D-sEVs for 1, 3, or 5 days.

Techniques: Cell Culture, Western Blot, Expressing, Confocal Microscopy, Labeling

Characterization of HAMA-CPC@3D-sEVs. ( A ) Release profiles of total protein and CD63 of sEVs in HAMA and HAMA-CPC. ( B ) Laser confocal observation of the growth morphology of HUVECs and BMSCs in HAMA and HAMA-CPC. ( C ) CCK-8 assay of HUVECs and BMSCs treated with HAMA and HAMA-CPC. ( D ) Live/Dead assay images and ( E ) quantitative analysis. ( F ) EdU assay images and ( G ) quantitative analysis

Journal: Journal of Nanobiotechnology

Article Title: Injectable HAMA-CPC hydrogels loaded with high-yield 3D bioprinted adipose-derived stem cell small extracellular vesicles for increased bone repair

doi: 10.1186/s12951-025-03596-4

Figure Lengend Snippet: Characterization of HAMA-CPC@3D-sEVs. ( A ) Release profiles of total protein and CD63 of sEVs in HAMA and HAMA-CPC. ( B ) Laser confocal observation of the growth morphology of HUVECs and BMSCs in HAMA and HAMA-CPC. ( C ) CCK-8 assay of HUVECs and BMSCs treated with HAMA and HAMA-CPC. ( D ) Live/Dead assay images and ( E ) quantitative analysis. ( F ) EdU assay images and ( G ) quantitative analysis

Article Snippet: Bone marrow stromal cells (BMSCs) (Solarbio, China) or human umbilical vein endothelial cells (HUVECs) (Gibco, USA) were seeded at a density of 1 × 104 cells/well in 96-well plates and cultured in the extract liquids of HAMA-CPC, HAMA-CPC@2D-sEVs, or HAMA-CPC@3D-sEVs for 1, 3, or 5 days.

Techniques: CCK-8 Assay, Live Dead Assay, EdU Assay